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Oroboros Instruments clark-type electrode selective for o2 oxygraph-2k
Clark Type Electrode Selective For O2 Oxygraph 2k, supplied by Oroboros Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/oxygraph++o2+electrode/oxygraph+2k/pm39189748-417-12-18
Average 90 stars, based on 1 article reviews
clark-type electrode selective for o2 oxygraph-2k - by Bioz Stars, 2026-10
90/100 stars

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Article Title: Chronic undernutrition impairs renal mitochondrial respiration accompanied by intense ultrastructural damage in juvenile rats.
Article Snippet: This study investigated whether chronic undernutrition alters the mitochondrial structure and function in renal proximal tubule cells, thus impairing fluid transport and homeostasis.. We previously showed that chronic undernutrition downregulates the renal proximal tubules (Na+K)ATPase, the main molecular machine responsible for fluid transport and ATP consumption.. Male rats received a multifactorial deficient diet, the so-called Regional Basic Diet (RBD), mimicking those used in impoverished regions worldwide, from weaning to a juvenile age (3 months).

Article Title: Changes in the mitochondrial phosphoproteome during mammalian hibernation.
Article Snippet: Respiration rates of purified liver mitochondria (25 l aliquot, 0.1 mg protein) were measured with a Clark-type polarographic O2 electrode (Oxygraph-2k; Oroboros, Innsbruck, Austria) in 2 ml (total volume) of mitochondrial respiration assay buffer [MiR05; 110 mM sucrose, 0.5 mM EGTA, 3 mM MgCl2, 60 mM K-lactobionate, 20 mM taurine, 10 mM KH2PO4, 20 mM HEPES, pH 7.1 at 30°C, 1% (wt/vol) BSA].

Article Title: Nitric oxide and superoxide anion production during heparin-induced capacitation in cryopreserved bovine spermatozoa.
Article Snippet: The aim of this work was to quantify NO, O2 ) and ONOO production during heparin-induced capacitation of cryopreserved bovine spermatozoa.. A time dependent hyperbolic increase was observed for heparin-dependent capacitation, O2 uptake, and NO production.. Conversely, O2 ) production was increased during the first 15 min of incubation, showing a decrease from this time until 45 min. At 15 min of heparin incubation, a threefold increase in O2 consumption (5.9 ± 0.6 nmol ⁄min · 10 cells), an enhancement in NO release (1.1 ± 0.2 nmol ⁄min · 10 cells), and a five-fold increase in O2 ) production (1.3 ± 0.07 nmol ⁄min · 10 cells), were observed.

Article Title: Early Effects of Extracellular Vesicles Secreted by Adipose Tissue Mesenchymal Cells in Renal Ischemia Followed by Reperfusion: Mechanisms Rely on the Restoration of the Redox Tissular Environment
Article Snippet: Oxygen consumption by isolated mitochondria from kidney proximal tubule cells We measured oxygen consumption (QO2) by isolated mitochondria using a high-resolution O2 electrode (Oxygraph-2K, OROBOROS Instruments, Innsbruck, Austria) at 37oC in the solution above descript for mitochondrial O2•- determination, except that the components required to detect H2O2 formation were omitted and 0.06 mg/mL mitochondrial protein was used.

Isolation:

Article Title: Extracellular vesicles from hypoxia-preconditioned mesenchymal stem cells preserve mitochondrial functions and redox homeostasis in ischemia-reperfusion-induced acute kidney injury.
Article Snippet: Acute kidney injury (AKI) induced by ischemia-reperfusion (I/R) remains a significant clinical challenge due to its rapid progression, limited therapeutic options, and high morbidity.. Mitochondrial dysfunction is a critical component of AKI pathogenesis, contributing to oxidative stress, impaired bioenergetics, and tissue injury.. Extracellular vesicles (EV) derived from mesenchymal stem cells (MSC) have emerged as potential candidates for organ protection through the modulation of inflammatory and oxidative pathways.

Activity Assay:

Article Title: Extracellular vesicles from hypoxia-preconditioned mesenchymal stem cells preserve mitochondrial functions and redox homeostasis in ischemia-reperfusion-induced acute kidney injury.
Article Snippet: Acute kidney injury (AKI) induced by ischemia-reperfusion (I/R) remains a significant clinical challenge due to its rapid progression, limited therapeutic options, and high morbidity.. Mitochondrial dysfunction is a critical component of AKI pathogenesis, contributing to oxidative stress, impaired bioenergetics, and tissue injury.. Extracellular vesicles (EV) derived from mesenchymal stem cells (MSC) have emerged as potential candidates for organ protection through the modulation of inflammatory and oxidative pathways.



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